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enormalstructureofcondylarcartilage, andthecellsofthesecartilagesactbiologically, sothecartilagesculturedwithinthisperiodcanbeusedasthesubjectstostudythephysiologyandpathologyofMCCinvitro. 1Keywords] mandibularcondylarcartilage; organculture * Foundation : 2003 Reserve of Yunnan Science and Technology Foundation (2003RC) ** Corresponding author: Zhang Min 0871,5381562,0871.5357627 E. mail: zhangmin2004926 @ sina. be separated from the body of the egg coin . The results showed that using 1mM and 0.1mM of IPTG induced the expression of no significant difference in the induction of 6h,[link widoczny dla zalogowanych], the highest expression , but was found with pre- induction time, the target protein degradation also increased , so the final choice protein Higher expression of 4h. SDS-PAGE electrophoresis in the supernatant of sonication has GbpD fusion protein , indicating that the expression of the fusion protein was soluble . And the inclusion bodies of expressed proteins compared to the expression of soluble form of the expression of protein although low ,[link widoczny dla zalogowanych], but it has a natural protein conformation and structure, with the function of the original activity of the protein ,[link widoczny dla zalogowanych], suitable for functional studies of proteins . By Ni-NTA affinity chromatography purified ,[link widoczny dla zalogowanych], SDS-PAGE analysis showed that was about 76kDa fusion protein , and the purity of more than 90% for the next step for antibody and protein function of the foundation . [
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