 Wysłany: Pon 15:20, 14 Mar 2011 Temat postu: mbt scarpe prezzi D Streptococcus mutans glucan bi |
|
| D Streptococcus mutans glucan binding protein gene cloning and prokaryotic expression and purification
enormalstructureofcondylarcartilage, andthecellsofthesecartilagesactbiologically, sothecartilagesculturedwithinthisperiodcanbeusedasthesubjectstostudythephysiologyandpathologyofMCCinvitro. 1Keywords] mandibularcondylarcartilage; organculture * Foundation : 2003 Reserve of Yunnan Science and Technology Foundation (2003RC) ** Corresponding author: Zhang Min 0871,5381562,0871.5357627 E. mail: zhangmin2004926 @ sina. be separated from the body of the egg coin . The results showed that using 1mM and 0.1mM of IPTG induced the expression of no significant difference in the induction of 6h,timberland scarpe, the highest expression , but was found with pre- induction time, the target protein degradation also increased , so the final choice protein Higher expression of 4h. SDS-PAGE electrophoresis in the supernatant of sonication has GbpD fusion protein , indicating that the expression of the fusion protein was soluble . And the inclusion bodies of expressed proteins compared to the expression of soluble form of the expression of protein although low ,clarks shoes outlet, but it has a natural protein conformation and structure, with the function of the original activity of the protein ,scarpe nike 2011, suitable for functional studies of proteins . By Ni-NTA affinity chromatography purified ,new balance outlet, SDS-PAGE analysis showed that was about 76kDa fusion protein , and the purity of more than 90% for the next step for antibody and protein function of the foundation . [ ,],mbt scarpe prezzi,[More articles related to topics： clarks shoes outlet X ray velopharyngeal anatomy M new balance outlet Acute superior mesenteric arter asics tiger shoes Vice parotid gland tumor report |
|
