Serum pharmacology research progress in Chinese me

px215467

Serum pharmacology research progress in Chinese medicine

Abstract serum pharmacology refers to the serum containing medicines in vitro reaction system applied to a semi-in vivo method. Has been widely used in the pharmacological effects of Chinese herbal compound and the mechanism of. This article discusses the method of serum pharmacology status and progress, combined with the author's ideas, on the Pharmacology of the prospect. Key words serum pharmacology; traditional Chinese medicine research; semi-vivo; evaluation; Outlook serum pharmacology is the Japanese scholar Hiroko Iwama [1] In 1984, the first and Institute of Chinese medicine was first proposed. 1988, Tokyo, Japan Shinichi Tashiro hospital [2] formally proposed the It refers to the Chinese or traditional Chinese medicine by mouth to animals fed animals after a certain time of blood collection, separation of serum, serum containing medicines in vitro experiments a semi-in vivo method. Chinese papers League finishing. 1990 years after the start of serum pharmacology of Chinese medicine in China by researchers in the attention. The method focuses on traditional Chinese medicine and the chemical composition of compound complex and diverse characteristics, instead of decoction containing serum or extracts from in vitro experiments, it has overcome the Chinese crude preparations in vitro directly the shortcomings of the credibility of the results was superior to traditional Chinese medicine preparations used directly for in vitro experiments crude. And it combines modern chemistry, microbiology, molecular biology, pharmacology, clinical and other subjects, serum pharmacological research methods continue to be used in some traditional Chinese medicine and western medicine pharmacology, and has made some progress. In this paper, serum pharmacology research progress in China, application, evaluation, and research prospects were reviewed. 1 the production of serum pharmacology pharmacology of Chinese herbal compound with the gradual, requiring researchers to raise the level of the cell, subcellular or molecular level, a lot of research needs carried out in vitro. Carried out in vitro tests, so studies of Chinese medicine to help a carrier. In 1984, the first and the Institute of Chinese Medicine, the Japanese scholar Hiroko Iwama presented to the animals fed with Chinese medicine, in a certain period of time, instead of with a decoction containing serum or extracts from the idea of in vitro experiments. Subsequently, the National Kyoto Hospital, Japan, Shinichi Tashiro, also in 1988 and 1992 the author, proposed the gradually carried out the research. 2 serum pharmacology Research in China 2.1 Aging Serum pharmacology relationship used to evaluate the efficacy of drug-containing serum, HE can be collected when the higher serum drug content, find the optimal blood sampling time after administration to avoid the consumption of the efficacy of metabolism or not false negative results caused by absorption is a very important issue. Since the exact efficacy of serum components is not clear, so often indicators to judge the efficacy of drug delivery and blood collection time, the merits of the time, the Researchers of different blood sampling time, administration time and efficacy were studied. administration time, the currently used methods are: 1 dose, or interval of 2 ~ 4 h administration, or 1 to 3 times per day, continuous 3 ~ 10 d. Ning-Sheng Wang [3] that, according to pharmacokinetic theory, the fixed delivery time interval, the same dose after repeated administration, plasma drug concentration to reach steady-state concentration, and at this time to obtain the concentration of different blood serum containing experiment showed high and similar to the effect intensity. Meng Li et al [4] Danshen in serum on neutrophil release of reactive oxygen species in study, preparation of serum containing experiments also confirmed this view. That the same dosage regimen for several days over a dose administration were significantly different, possibly drug-induce endogenous bioactive substances [5]. This conclusion has been reached a consensus. blood collection time, the ideal blood sampling time should be the peak plasma concentration. A pure Mongolian [6] that the physical characteristics of the experimental animal digestion and absorption of Chinese medicine, bio-transformation of blood sampling time to determine the scope of features is very reasonable. Such as rats usually 1 ~ 2 h up to the peak plasma concentration, when the serum is the drug-containing serum; time extension will be transformed, some medicines, a drug metabolism serum. Generally more concentrated in the blood sampling time after the last administration between 30 min to 3 h. After the administration of traditional Chinese medicine, different drugs with different peak time, it is difficult for a unified standard blood collection time. Such as Zhou Ming Mei et al [7] found that the first 30 min after administration of the wind Drink the inhibition of platelet 5-HT release after administration of other times than the role of strong, made 30 min after administration of blood is the best time. Li Yi-Kui [8] The peak time of 96 drugs made after statistics 1 ~ 2 h after the drug blood is more reasonable. Package Jinfeng et al [9] that the response to different times pre-blood test, the serum at different times were compared with blood sampling time and then determine the most reliable and scientific method. However, Li Yi-Kui in a large number of traditional Chinese medicine and other pharmacokinetic parameters were analyzed, the program presents a passage, a day after 2 consecutive 3 d, 1 h after the last administration blood, known as the This will help save money, manpower and time. 2.2 serum pharmacological dose-effect relationship of dose has not been determined, and its size depends on the amount of: First, the amount of animal drug in To some extent, the greater the amount of serum administered in higher drug content; Second, the reaction system or culture system containing serum concentration, the higher the serum concentration of the larger dose. Containing serum in vitro but added after the reaction, the concentration of diluted, so that drug concentration reach reaction conditions in vivo drug concentrations. Wang Liqian et al [11] proposed using the amount of times the clinical dose is equal to animal body surface area index, multiplied by the medium within the calculated dilution of serum preparation containing serum dose is more reasonable. Studies have shown that drug-containing serum from animals administered dose and serum concentration of the amount of joint decision, because the serum concentration of culture system changes is limited (high serum concentration of cells will produce toxic effects in the study of drug-containing Serum inhibition of cell proliferation or promoting apoptosis, it may come to false positive results). Yang Yanfang [12] that should be clinically equivalent dose basis, pay attention to drug concentration, for high doses (such as clinical equivalent doses of 5 times, 10 times) obtained positive results, should be careful in making conclusions or stay there is room. Therefore, the concentration of serum containing relatively fixed, by changing the animals to adjust the amount of drugs administered serum drug content, is a more realistic approach. 3 serum pharmacological experiments in our science research 3.1 serum pharmacological experiments in the development of Chinese drug current , the main research and development of Chinese medicine is to find drugs active ingredients or parts to prepare refined traditional chinese medicine, improve the clinical efficacy and reduced side effects. Method of serum pharmacology experiments established that the realization of this goal becomes more feasible. Mainly in the following aspects: ① in vitro pharmacological experiments, further validation and evaluation of drug efficacy, the use of effective drugs containing serum and / or active ingredients of drugs for initial screening; ② drug serum chemistry and serum pharmacology , Analytical Chemistry of Chinese medicine, Chinese medicine pharmacokinetics, especially the gastrointestinal pharmacokinetics of traditional Chinese medicine, herbal medicine serum pharmacokinetics, and Treatment of Chinese medicine and other Pharmacokinetics of the combination will be beneficial to drug active ingredients or parts of the search and identification; ③ on comparison of drug preparation and selection process; ④ mechanism of action of drugs to do more research. 3.2 serum pharmacology pharmacokinetics in the recipe (PK) of the application yellow seats and so will the Treatment prescription pharmacokinetics of the compound in the revised prescription compound pharmacokinetics Effect of composition dynamics hypothesis [13]. The prescription is to explore the serum target component elements of compound material basis for the efficacy of [14]. Not only that, but also of the target components in the body of the concentration-time curve, mathematical model,[link widoczny dla zalogowanych], the PK parameters can be directly guide clinical treatment, the therapeutic drug monitoring (TDM), which will effectively improve the efficacy, so simply explore the material basis for efficacy prescription depth and development. The serum pharmacology [15] studied the process of taking the prescription containing serum after in vitro pharmacodynamic experiments, by exploring the effects associated with the prescriptions set out the composition of indirect pharmacodynamic substances. 3.3 serum pharmacology application in cancer research vitro anti-tumor mechanism of Chinese herbal medicine is an important method. For the anti-tumor compound traditional Chinese medicine for a variety of traditional Chinese medicine in their serum compatibility of the active ingredient in the detection of quantitative analysis of the progress has not been a breakthrough, the serum pharmacological anti-tumor compound is an effective way of .